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Epub 2016 Jul 25

Fecal samples were homogenized by adding methanol

One anomaly from previous measurements was the high intracellular steady-state oxidation of roGFP2 for fusion contructs with ScGrx7, in particular, when a WP-motif or a loop from class II glutaredoxins was introduced

| Concentration | BAC Water | Doses at 1.0mg | Doses at 2.4mg | |---------------|-----------|----------------|----------------| | 5mg/mL | 2.0mL | 10 doses | ~4 doses | | 2.5mg/mL | 4.0mL | 10 doses | ~4 doses | **Best practice:** 2.0mL BAC water gives a clean 5mg/mL concentration

It is a powerful antioxidant naturally present in the body, crucial for combating oxidative stress

2,134,152 Conversely, PGC-1 mutation impairs the transcription of TFAM, resulting in dysfunctional mtDNA replication